BD566-CA (SC220)

BD566-CA is a bright, fluorogenic, cell permeable and orange emitting ChloroAlkane (CA) substrate for Halo-tag labeling. Contains 1 vial of BD566-CA (10 nmol) in lyophilised form allowing to prepare 50 ul of 1000x DMSO stock solution (100 stainings). BD566-CA (also named BD566-HTL) is a ChloroAlkane (CA) derivative of the bridged-bicycle-strengthened orange fluorophore BD566. The unique BriDye™ bicyclic ether bridge technology1) confers to BD566-CA an exceptional photostability. It emits light in the orange part of the UV-ViS spectrum, it is highly cell permeable and well suited for superresolution imaging. BD566-CA absorption and emission maxima are 566 nm and 594 nm, respectively with an extinction coefficient of 108 000 M⁻¹ cm⁻¹ and a quantum yield of 0.85 (HT7 bound values). BD566-CA is highly cell-permeable, highly photostable, and compatible with standard Cy3 or TMR filter settings for widefield, confocal, SIM, or STED imaging in living or fixed specimens. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag. Upon reaction with a CA derivative, Halo-tag forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag fusion.

• Size: 100 stainings
• Storage: -20°C
• Shipping: room temperature
• Category: ChloroAlkane substrates