STAT6; Signal transducer and activator of transcription 6; IL-4 Stat
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human STAT6 around the phosphorylation site of Tyr641. AA range:608-657
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
94kD
GeneID（Human）:
6778
Human Swiss-Prot No:
P42226
Cellular localization:
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Background:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010],
Western blot analysis of Stat6 (phospho Tyr641) Polyclonal Antibody, using Hela,Jurkat cell treated or untreated with IFN 2500U/ml 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.
Western Blot analysis of various cells using Phospho-Stat6 (Y641) Polyclonal Antibody
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT6 (Phospho-Tyr641) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT6 (Phospho-Tyr641) Antibody. The picture on the right is blocked with the phospho peptide.