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iba-lifesciences
SKU(재고 관리 코드):2-1201-010
Strep-Tactin® Sepharose® resin
Strep-Tactin® Sepharose® resin
Strep-Tactin® Sepharose® 50 % suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The resin is particularly suited for the purification of proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins because of the increased affinity. It can be used for the purification of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly binds biotin, so if free biotin is expected use BioLock. The suspension format allows the packing of gravity flow columns with custom resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.
Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to a low pressure stability.
For information on our other resins, please check the resin specifications.
Specifications
| Bead Size: | 45 - 165 µm |
|---|---|
| Biotin Binding Capacity: | > 300 nmol/ml resin |
| Exclusion Limit: | 3 x 10^7 |
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Strep-tag®II Binding Capacity: | 3 mg/ml |
| Support: | 4% agarose |
Shipping information
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
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