TFEB Polyclonal Antibody

Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network. TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the up-regulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy. TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in up-regulation of autophagosomes and lysosomes. Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress. During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes. TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB.

The TFEB Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and quantification of Transcription Factor EB (TFEB) protein in various biological samples. This antibody is produced using advanced immunization techniques, ensuring high affinity and specificity towards TFEB.

TFEB is a crucial transcription factor involved in the regulation of autophagy, lysosomal biogenesis, and cellular clearance processes. Its dysregulation has been implicated in various diseases, including neurodegenerative disorders, cancer, and metabolic disorders. Therefore, accurate measurement of TFEB protein levels is essential for understanding its role in these pathological conditions.

The TFEB Polyclonal Antibody exhibits exceptional performance in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It recognizes both endogenous and recombinant TFEB protein, enabling researchers to study TFEB expression and localization in different cell types and tissues.

This antibody is manufactured under stringent quality control measures to ensure batch-to-batch consistency and reproducibility. It has been extensively validated in multiple experimental settings, demonstrating its reliability and accuracy. Additionally, the TFEB Polyclonal Antibody is provided in a convenient format, allowing for easy handling and storage.

In summary, the TFEB Polyclonal Antibody is a reliable and versatile tool for investigating the role of TFEB in various biological processes and disease states. Its high specificity, sensitivity, and ease of use make it an invaluable asset for researchers in the fields of cell biology, molecular biology, and biomedical research.