Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Tyrosine Hydroxylase. AA range:41-90
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
55kD
GeneID（Human）:
7054
Human Swiss-Prot No:
P07101
Cellular localization:
Cytoplasm, perinuclear region . Nucleus . Cell projection, axon . Cytoplasm . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle . When phosphorylated at Ser-19 shows a nuclear distribution and when phosphorylated at Ser-31 as well at Ser-40 shows a cytosolic distribution (By similarity). Expressed in dopaminergic axons and axon terminals. .
Background:
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using TH Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by i
Immunofluorescence analysis of HUVEC cells, using Tyrosine Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Tyrosine Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.