Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human TISB. AA range:58-107
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
36kD
GeneID（Human）:
677
Human Swiss-Prot No:
Q07352
Cellular localization:
Nucleus . Cytoplasm . Cytoplasmic granule . Cytoplasm, P-body . Shuttles between the nucleus and the cytoplasm in a XPO1/CRM1-dependent manner (By similarity). Component of cytoplasmic stress granules (PubMed:15967811). Localizes in processing bodies (PBs) (PubMed:17369404). .
Background:
This gene is a member of the TIS11 family of early response genes, which are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. This gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
Western Blot analysis of various cells using TIS11B Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TISB Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat, HeLa, A549, and LOVO cells, using TISB Antibody. The lane on the right is blocked with the synthesized peptide.