TMEM185A; CXorf13; FAM11A; Transmembrane protein 185A; Protein FAM11A
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human TMEM185A. AA range:290-339
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
41kD
GeneID（Human）:
84548
Human Swiss-Prot No:
Q8NFB2
Cellular localization:
Cell projection, dendrite . Membrane ; Multi-pass membrane protein .
Background:
The protein encoded by this gene is predicted to be a transmembrane protein. This gene is best known for localizing to the CpG island of the fragile site FRAXF. The 5' untranslated region of this gene contains a CGG trinucleotide repeat sequence that normally consists of 7-40 tandem CGG repeats but which can expand to greater than 300 repeats. Methylation of the CpG island leads to transcriptional silencing of this gene, but neither the silencing nor an expanded repeat region appear to manifest itself in a clear phenotypic manner. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Aug 2013],
Immunofluorescence analysis of MCF7 cells, using TMEM185A Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, using TMEM185A Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HT-29 cells using TMEM185A antibody.
Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200