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PROTEINTECH
SKU(재고 관리 코드):13343-1-AP
TPSAB1 Polyclonal antibody
TPSAB1 Polyclonal antibody
Tested Applications
| Positive WB detected in | A431 cells, K-562 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human tonsillitis tissue, human appendix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse skin tissue, HepG2 cells |
| Positive FC detected in | U-937 cells |
Recommended dilution
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
13343-1-AP targets TPSAB1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TPSAB1 fusion protein Ag4167 |
| Full Name | tryptase alpha/beta 1 |
| Calculated Molecular Weight | 275 aa, 31 kDa |
| Observed Molecular Weight | 32-38 kDa |
| GenBank Accession Number | BC028059 |
| Gene Symbol | TPSAB1 |
| Gene ID (NCBI) | 7177 |
| RRID | AB_2206197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| PRODUCT SPECIFIC PROTOCOLS | |
|---|---|
| WB protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| IHC protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| IF protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| IP protocol for TPSAB1 antibody 13343-1-AP | Download protocol |
| STANDARD PROTOCOLS | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| SPECIES | APPLICATION | TITLE |
|---|---|---|
| human | IF |
Clin Transl Med Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour |
| rat | IHC |
Front Immunol Palmatine treats urticaria by reducing inflammation and increasing autophagy |
| human | IHC |
IUBMB Life Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma. |
| human | WB |
BMC Cancer The release of tryptase from mast cells promote tumor cell metastasis via exosomes. |
| mouse | WB,IF |
Int J Mol Sci An Amide Alkaloid Isolated from Ephedra sinica Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation |
| mouse | IF |
Ann Transl Med Identification of hub genes and infiltrating immune cells in skeletal muscle in obesity |
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