Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human TRHR. AA range:195-244
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
50kD
GeneID（Human）:
7201
Human Swiss-Prot No:
P34981
Cellular localization:
Cell membrane ; Multi-pass membrane protein .
Background:
This gene encodes a G protein-coupled receptor for thyrotropin-releasing hormone (TRH). Upon binding to TRH, this receptor activates the inositol phospholipid-calcium-protein kinase C transduction pathway. Mutations in this gene have been associated with generalized thyrotropin-releasing hormone resistance. [provided by RefSeq, Sep 2011],
Immunofluorescence analysis of A549 cells, using TRHR Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells, using TRHR Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using TRHR antibody.