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Tosoh Bioscience LLC

0005102

TSKgel G2000SW

TSKgel G2000SW

As the first generation of silica-based SEC columns, TSKgel G2000SW columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.

TSKgel G2000SW columns are available in 10 and 13 μm for analytical and semi-preparative purifications.

 

Product Attributes

Base material: Silica
Particle size (mean): 10 µm, 13 µm
Pore size (mean): 12.5 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 5,000 - 1.0 × 105 Da
(globular proteins)


 
          

Calibration Curves for Globular Proteins, Polyethylene Oxides and Dextrans
for TSKgel SW Columns

SEC_G2000SW-Calibration-curves-rev.png

Columns: TSKgel SW columns, 7.5 mm ID × 60 cm × 2
Mobile phase: dextrans and polyethylene oxides: distilled water;
proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm and RI
Temperature: A. 25°C  B. 25°C  C. 40°C
Samples: proteins (black circle),
polyethylene oxides (white circle),
dextrans (half circle)


Applications 

Separation of Nucleic Acid

SEC_G2000SW-Fig6rev.png

 

 


Column: TSKgel G2000SW, 10 µm,
7.5 mm ID × 60 cm
Mobile phase: acetic acid/triethylamine/H2O = 3/3/94
Flow rate: 0.74 mL/min
Detection: UV @ 260 nm
Sample: 1. uridine
2. uracil
3. thymine
4. adenosine
5. adenine

Comparison of the Pore Sizes of TSKgel SW Packings for the Separation of a Protein Mixture

SEC_G2000SW_fig1_rev.png

Columns: TSKgel SW columns, two 7.5 mm ID × 60 cm columns in series
Mobile phase: 0.3 mol/L NaCl in 0.05 mol/L phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Samples: 1. thyroglobulin, 2. bovine serum albumin, 3. β-lactoglobulin, 4. myoglobin, 5. cytochrome C, 6. glycine tetramer
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