TYR; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Applications:
WB;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Tyrosinase. AA range:471-520
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
80kD
GeneID（Human）:
7299
Human Swiss-Prot No:
P14679
Cellular localization:
Melanosome membrane ; Single-pass type I membrane protein . Melanosome . Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38. .
Background:
tyrosinase(TYR) Homo sapiens The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008],
Western Blot analysis of various cells using TYR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western Blot analysis of COS7 cells using TYR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Western blot analysis of lysates from COS7 cells, treated with UV 30', using Tyrosinase Antibody. The lane on the right is blocked with the synthesized peptide.