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ELK Biotechnology
SKU(재고 관리 코드):EM1004
V5-Tag Mouse Monoclonal Antibody
V5-Tag Mouse Monoclonal Antibody
V5-Tag Mouse Monoclonal Antibody
Overview
| Product name: | V5-Tag Mouse mAb |
| Reactivity: | N/A |
| Alternative Names: | N/A |
| Source: | Mouse |
| Applications: | WB, IP, IF |
| Species: | N/A |
| Species: | N/A |
| Recommended dilutions: | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | N/A |
| GeneID(Human): | N/A |
| Human Swiss-Prot No: | N/A |
| Cellular localization: | N/A |
| Background: | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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IP antibody use:5ug V5 Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G ag -
IF analysis of 293 cells transfected with a V5-tag protein, using ELKbio anti-V5Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-V5) -
1ug V5 fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000
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