VEGF-A Polyclonal Antibody

VEGFA (vascular endothelial growth factor A) is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of VEGFA in mice resulted in abnormal embryonic blood vessel formation. VEGFA is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of VEGFA have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

The VEGF-A Polyclonal Antibody is a highly specific and sensitive immunological reagent designed for the detection and quantification of Vascular Endothelial Growth Factor A (VEGF-A) in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human VEGF-A. The resulting polyclonal antibody exhibits high affinity and specificity towards VEGF-A, making it an ideal tool for research applications in the fields of angiogenesis, cancer biology, and vascular biology.

The VEGF-A Polyclonal Antibody is suitable for use in a variety of immunoassays, including Western blotting, ELISA, immunohistochemistry, and immunofluorescence. It has been validated for use in human, mouse, and rat samples, and has been shown to detect both the pro- and mature forms of VEGF-A. This antibody is supplied as a purified IgG fraction, and has been tested for its ability to recognize endogenous VEGF-A in various cell lines and tissues.

In summary, the VEGF-A Polyclonal Antibody is a reliable and versatile tool for the detection and quantification of VEGF-A in biological samples. Its high specificity and sensitivity make it an ideal reagent for researchers studying angiogenesis, cancer biology, and vascular biology.